Monday, 30 April 2012

Test Tube Baby Procedure India

Anything which occurs outside the body is called in vitro and when fertilization of egg with sperm occurs outside the body it is called "In Vitro Fertilization". The first In Vitro fertilization was done in the test tube and that is why it is popularly known as Test tube Baby.

Test tube baby process or Infertility IVF or IVF-In Vitro Fertilization is the one which is conceived outside the woman's womb.The sperm of man and the egg taken from the woman's ovaries are fertilised within a test tube in the laboratory. Once successful fertilisation is completed, the embryo is then transplanted into the woman's uterus to complete the pregnancy. The embryo won't survive within the test tube and needs to be transplanted to the woman's uterus for its normal growth.Normally this procedure is a part of the IVF (in vitro fertilisation) technique and is done in patients who have problems conceiving normally. 
The process of fertilization which normally in fallopian tube of the woman and the fertilized egg than moves into the uterus of the woman. It an implants there in to the uterus and developed into the baby. When for some reason this process of fertilization cannot take place naturally or sperm and egg cannot be transported naturally to the tube or from the tube to the uterus or either is egg having some problem or the sperm.
For whom test tube baby procedure is done?
If the female partner has both the fallopian tubes blocked or the female is suffering from severe endometriosis or the husband is very small number of motile and normal sperms or any other reason by which the fertilization which occurs in outer part of the fallopian tube does not take place.

Ovarian hyper stimulation
In order to boost-up IVF process there are two main protocols to stimulate the ovary. The long protocol involves down regulation (suppression or exhaustion) of the pituitary ovarian axis by the prolonged use of a GnRH . Whereas the short protocol jumps over the down regulation, consist of a procedure of fertility medications to stimulate the development of multiple follicles of the ovaries.
Egg retrieval
When the ovarian follicles have reached a certain degree of development, final maturation is stimulated, usually by an injection of human chorionic gonadotropin (hCG).This is commonly known as the "trigger shot. Then, hCG acts as an analogue of luteinising hormone, and ovulation would occur between 38 and 40 hours after a single hCG injection, but the egg recovered is performed at a time usually between 34 and 36 hours after hCG injection. This is just prior to when the follicles would split. This avails for scheduling the egg recovery procedure at a time when the eggs are fully mature.
Egg and sperm preparation
The treatment continues in the laboratory, the identified eggs are uncovered from surrounding cells and prepared for fertilization. An oocyte selection may be performed prior to fertilization to select eggs with best chances of successful and healthy pregnancy. At the same time, semen is made ready for fertilization by removing inactive cells and seminal fluid in a process called sperm washing. If semen is being provided by a sperm donor, it will usually have been prepared for treatment before being frozen and quarantined, and it will be soften to use.                                                        
Embryo culture
Culture of embryos can either be performed in an artificial culture medium or in an autologous endometrial co culture (on top of a layer of cells from the woman's own uterine lining). With artificial culture medium, there can either be the same culture medium throughout the period, or a sequential system can be used, in which the embryo is sequentially placed in different media.
There is a grading method developed by the laboratory to judge and select the Embryo quality. In order to have best output on pregnancy rates, it is clearly visible that a morphological scoring system is the method for selection of embryos.    
The Embryologist select the embryos based on the number of cells it possess, The age of the woman and other health and diagnostic factors would depend the number available in the cells.

Srishti, Assisted Fertility & Advanced Laparoscopy Center (SAFAL), Puducherry,India provides for all aspects of sub fertility diagnosis, treatment and laboratory services that you may require for assisted reproduction.Srishti is Pondicherry's first fertility center and Test tube baby centre.

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