Saturday, 28 April 2012

In Vitro Fertilization (IVF)- Best Solution for Infetility


In vitro fertilization-IVF or testtube baby process is the process by which eggs are removed from your ovaries and mixed with sperm in a laboratory culture dish. Fertilisation takes place in this dish, "in vitro", which means "in glass".

IVF treatment is likely to be recommended for the following fertility problems:
  • If you have blocked or damaged fallopian tubes.
  • If your partner has a minor problem with his sperm. Major problems are better treated using ICSI.
  • If you have tried fertility drugs, such as clomiphene, or another fertility treatment such as IUI, without success.
  • If you have been trying to conceive for at least two years, or less if you are 40 years or more. This is if a cause hasn't been found to explain why you have not become pregnant.

IVF treatment is one of major and effective treatments in infertility when other methods of assisted reproductive technology have failed. The stage of treatment involves hormonally controlling the ovulatory process, removing ova (eggs) from the woman's ovaries and allowing sperm fertilize them in a fluid medium. The fertilized egg (zygote) is then passed to the patient's uterus with the intent to establish a successful and healthy pregnancy.

In order to boost-up IVF process there are two main protocols to stimulate the ovary. The long protocol involves down regulation (suppression or exhaustion) of the pituitary ovarian axis by the prolonged use of a GnRH . Whereas the short protocol jumps over the down regulation, consist of a procedure of fertility medications to stimulate the development of multiple follicles of the ovaries.
Egg retrieval
When the ovarian follicles have reached a certain degree of development, final maturation is stimulated, usually by an injection of human chorionic gonadotropin (hCG).This is commonly known as the "trigger shot. Then, hCG acts as an analogue of luteinising hormone, and ovulation would occur between 38 and 40 hours after a single hCG injection, but the egg recovered is performed at a time usually between 34 and 36 hours after hCG injection. This is just prior to when the follicles would split. This avails for scheduling the egg recovery procedure at a time when the eggs are fully mature.
Egg and sperm preparation
The treatment continues in the laboratory, the identified eggs are uncovered from surrounding cells and prepared for fertilization. An oocyte selection may be performed prior to fertilization to select eggs with best chances of successful and healthy pregnancy. At the same time, semen is made ready for fertilization by removing inactive cells and seminal fluid in a process called sperm washing. If semen is being provided by a sperm donor, it will usually have been prepared for treatment before being frozen and quarantined, and it will be soften to use. 
 Embryo culture
Culture of embryos can either be performed in an artificial culture medium or in an autologous endometrial co culture (on top of a layer of cells from the woman's own uterine lining). With artificial culture medium, there can either be the same culture medium throughout the period, or a sequential system can be used, in which the embryo is sequentially placed in different media.
Embryo selection
There is a grading method developed by the laboratory to judge and select the Embryo quality. In order to have best output on pregnancy rates, it is clearly visible that a morphological scoring system is the method for selection of embryos.
Embryo transfer
The Embryologist select the embryos based on the number of cells it possess, The age of the woman and other health and diagnostic factors would depend the number available in the cells.

For more details about testtube baby procedure,


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